Zhx2^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zhx2^{tm1d(KOMP)Wtsi}
• Name: zinc fingers and homeoboxes 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6284310
• Gene: Zhx2 Location: Chr15:57558063-57703228 bp, + strand Genetic Position: Chr15, 24.01 cM
• Alliance: Zhx2^{tm1d(KOMP)Wtsi} page
• IMPC: Zhx2 gene page

Mutation origin

• Mutant Cell Line: EPD0245_1_A06
• Germline Transmission: Earliest citation of germline transmission: J:242505
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 57683948 of Chromosome 15 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 57687497. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:242505)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zhx2 Mutation: 35 strains or lines available

References

• Original: J:242505 Jiang J, et al., Zhx2 (zinc fingers and homeoboxes 2) regulates major urinary protein gene expression in the mouse liver. J Biol Chem. 2017 Apr 21;292(16):6765-6774
• All: 1 reference(s)