Mtdh^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mtdh^{tm1c(KOMP)Wtsi}
• Name: metadherin; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6281604
• Gene: Mtdh Location: Chr15:34082613-34143683 bp, + strand Genetic Position: Chr15, 13.98 cM, cytoband B3.3
• Alliance: Mtdh^{tm1c(KOMP)Wtsi} page
• IMPC: Mtdh gene page

Mutation origin

• Mutant Cell Line: EPD0291_7_A08
• Germline Transmission: Earliest citation of germline transmission: J:250419
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 34113683 of Chromosome 15 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon at position 34114454. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:250419)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mtdh Mutation: 33 strains or lines available

References

• Original: J:250419 Meng X, et al., Genetic Deficiency of Mtdh Gene in Mice Causes Male Infertility via Impaired Spermatogenesis and Alterations in the Expression of Small Non-coding RNAs. J Biol Chem. 2015 May 8;290(19):11853-64
• All: 3 reference(s)