Hspb6^em1Gcfa
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hspb6^em1Gcfa
• Name: heat shock protein, alpha-crystallin-related, B6; endonuclease-mediated mutation 1, Guo-Chang Fan
• MGI ID: MGI:6281044
• Gene: Hspb6 Location: Chr7:30251961-30254868 bp, + strand Genetic Position: Chr7, 18.56 cM
• Alliance: Hspb6^em1Gcfa page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/Cas9 technology was used to inject 4 gRNAs, two gRNAs targeting exon 1 and the other two to exon 2. Sequence between the gRNA targeting sites was deleted. Western blot analysis confirmed absence of protein. (J:271250)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hspb6 Mutation: 9 strains or lines available

References

• Original: J:271250 Peng J, et al., An Hsp20-FBXO4 Axis Regulates Adipocyte Function through Modulating PPARgamma Ubiquitination. Cell Rep. 2018 Jun 19;23(12):3607-3620
• All: 1 reference(s)