Lrit1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Lrit1^{tm1c(EUCOMM)Hmgu}
• Name: leucine-rich repeat, immunoglobulin-like and transmembrane domains 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6281021
• Synonyms: Lrit1^fl
• Gene: Lrit1 Location: Chr14:36776787-36786903 bp, + strand Genetic Position: Chr14, 21.29 cM
• Alliance: Lrit1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Lrit1 gene page

Mutation origin

• Mutant Cell Line: HEPD0832_7_G03
• Germline Transmission: Earliest citation of germline transmission: J:271244
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 36778762 of Chromosome 14 upstream of the critical exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 36779917. The critical exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon 2. (J:271244)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lrit1 Mutation: 37 strains or lines available

References

• Original: J:271244 Ueno A, et al., Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity. Cell Rep. 2018 Mar 27;22(13):3548-3561
• All: 1 reference(s)