Atg3^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Atg3^{tm1c(EUCOMM)Hmgu}
• Name: autophagy related 3; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6274408
• Synonyms: Atg3^flox, Atg3flox
• Gene: Atg3 Location: Chr16:44979192-45008901 bp, + strand Genetic Position: Chr16, 29.45 cM, cytoband B5
• Alliance: Atg3^{tm1c(EUCOMM)Hmgu} page
• IMPC: Atg3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269255
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt0 cassette was inserted at position 44986911 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 44987639. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:269255)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Atg3 Mutation: 19 strains or lines available

References

• Original: J:269255 Bharath LP, et al., Endothelial Cell Autophagy Maintains Shear Stress-Induced Nitric Oxide Generation via Glycolysis-Dependent Purinergic Signaling to Endothelial Nitric Oxide Synthase. Arterioscler Thromb Vasc Biol. 2017 Sep;37(9):1646-1656
• All: 4 reference(s)