Gls^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Gls^{tm1c(KOMP)Mbp}
• Name: glutaminase; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:6274228
• Synonyms: GLS1^flox, GLS^fl
• Gene: Gls Location: Chr1:52202607-52272391 bp, - strand Genetic Position: Chr1, 26.86 cM
• Alliance: Gls^{tm1c(KOMP)Mbp} page
• IMPC: Gls gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269124
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 52235077 of Chromosome 1 upstream of the critical exons 10 and 11 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 10 and 11 at position 52236375. The critical exons 10 and 11 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exons 10 and 11. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:269124, J:321423)

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gls Mutation: 42 strains or lines available

References

• Original: J:269124 Johnson MO, et al., Distinct Regulation of Th17 and Th1 Cell Differentiation by Glutaminase-Dependent Metabolism. Cell. 2018 Dec 13;175(7):1780-1795.e19
• All: 4 reference(s)