Comt^tm1.1Tunb
Targeted Allele Detail

Summary

• Symbol: Comt^tm1.1Tunb
• Name: catechol-O-methyltransferase; targeted mutation 1.1, Elizabeth Tunbridge
• MGI ID: MGI:6274226
• Synonyms: COMT-Met
• Gene: Comt Location: Chr16:18225636-18245602 bp, - strand Genetic Position: Chr16, 11.4 cM
• Alliance: Comt^tm1.1Tunb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269121
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: The targeting was designed to alter the DNA sequence of codon 148 in exon 3 from CTA to ATG creating a leucine-to-methionine amino acids substitution (L148M) and insert a loxP-flanked PGK-neo selection cassette in intron 3. The substitution is analogous to the human Val<158>Met mutation. Cre-mediated recombination removed the floxed neo cassette. Immunoblot analysis indicats that both soluble and membrane bound protein isoforms are reduced in all brain regions. (J:269121)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Comt Mutation: 23 strains or lines available

References

• Original: J:269121 Barkus C, et al., Genotype-Dependent Effects of COMT Inhibition on Cognitive Function in a Highly Specific, Novel Mouse Model of Altered COMT Activity. Neuropsychopharmacology. 2016 Dec;41(13):3060-3069
• All: 1 reference(s)