Crisp4^tm1.1Osb
Targeted Allele Detail

Summary

• Symbol: Crisp4^tm1.1Osb
• Name: cysteine-rich secretory protein 4; targeted mutation 1.1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6274002
• Synonyms: Crisp4^tm1.1Pasc
• Gene: Crisp4 Location: Chr1:18185415-18216126 bp, - strand Genetic Position: Chr1, 5.65 cM, cytoband A4
• Alliance: Crisp4^tm1.1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269106
• Parent Cell Line: EGR-G101 (ES Cell)
• Strain of Origin: C57BL/6NSlc-Tg(CAG-EGFP,Acr-EGFP)2Osb

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Using IKMC targeting vector PRPGS00028_A_A10, a lacZ gene expression cassette and a loxP site flanked neomycin resistance gene cassette were inserted into intron 4. A third loxP site was inserted into intron 5. The neo cassette and exon 5 were deleted through subsequent cre-mediated recombination, leaving a reporter knockout allele. (J:269106)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crisp4 Mutation: 23 strains or lines available

Notes

Used IKMC targeting vector PRPGS00028_A_A10.

References

• Original: J:269106 Carvajal G, et al., Impaired male fertility and abnormal epididymal epithelium differentiation in mice lacking CRISP1 and CRISP4. Sci Rep. 2018 Dec 3;8(1):17531
• All: 1 reference(s)