Tnfsf11^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tnfsf11^{tm1c(EUCOMM)Wtsi}
• Name: tumor necrosis factor (ligand) superfamily, member 11; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6273217
• Synonyms: Tnfsf11^fl
• Gene: Tnfsf11 Location: Chr14:78514886-78545483 bp, - strand Genetic Position: Chr14, 41.26 cM
• Alliance: Tnfsf11^{tm1c(EUCOMM)Wtsi} page
• IMPC: Tnfsf11 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267632
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 78521435 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 78522291. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:267632)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tnfsf11 Mutation: 30 strains or lines available

References

• Original: J:267632 Bando JK, et al., The Tumor Necrosis Factor Superfamily Member RANKL Suppresses Effector Cytokine Production in Group 3 Innate Lymphoid Cells. Immunity. 2018 Jun 19;48(6):1208-1219.e4
• All: 1 reference(s)