Chd2^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Chd2^{tm1c(EUCOMM)Hmgu}
• Name: chromodomain helicase DNA binding protein 2; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6273206
• Synonyms: Chd2-flox
• Gene: Chd2 Location: Chr7:73076400-73191494 bp, - strand Genetic Position: Chr7, 41.85 cM
• Alliance: Chd2^{tm1c(EUCOMM)Hmgu} page
• IMPC: Chd2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268688
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 73168819 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 73170006. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:268688)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chd2 Mutation: 187 strains or lines available

References

• Original: J:268688 Kim YJ, et al., Chd2 Is Necessary for Neural Circuit Development and Long-Term Memory. Neuron. 2018 Dec 5;100(5):1180-1193.e6
• All: 1 reference(s)