Syne1^em2Didh
Endonuclease-mediated Allele Detail

Summary

• Symbol: Syne1^em2Didh
• Name: spectrin repeat containing, nuclear envelope 1; endonuclease-mediated mutation 2, Didier Hodzic
• MGI ID: MGI:6272747
• Synonyms: Nes1^FLAG
• Gene: Syne1 Location: Chr10:4970917-5501482 bp, - strand Genetic Position: Chr10, 2.01 cM, cytoband A1
• Alliance: Syne1^em2Didh page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag)
• Mutation: Insertion
• Mutation details: Exon -1 (the last exon) was targeted for the insertion of a 66 bp sequence with an sgRNA and a template oligonucleotide using CRSIPR/Cas9 technology. The inserted sequence codes for three FLAG epitopes and was inserted just before the stop codon. This creates an allele where isoforms that incorporate this exon and translate it in this particular reading frame have a C-terminal FLAG tag. (J:268589)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Syne1 Mutation: 202 strains or lines available

References

• Original: J:268589 Potter C, et al., The KASH-containing isoform of Nesprin1 giant associates with ciliary rootlets of ependymal cells. Neurobiol Dis. 2018 Jul;115:82-91
• All: 1 reference(s)