Cdk1^em2Nagy
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cdk1^em2Nagy
• Name: cyclin dependent kinase 1; endonuclease-mediated mutation 2, Andras Nagy
• MGI ID: MGI:6272331
• Synonyms: Cdk1-TK
• Gene: Cdk1 Location: Chr10:69170976-69188742 bp, - strand Genetic Position: Chr10, 36.07 cM
• Alliance: Cdk1^em2Nagy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268168
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Endonuclease-mediated (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The 3' end of the gene was targeted with a targeting vector and sgRNAs using CRISPR/Cas9 technology. The vector that was inserted just before the stop codon contained the following elements: sequence for the F2A self-cleaving peptide, a loxP site, a neomycin resistance gene cassette, a second loxP site, a modified herpes simplex virus thymidine kinase gene (TK.007), sequence for the T2A self-cleaving peptide, and the mCherry red fluorescent protein marker gene. The neo cassette was removed through subsequent cre-mediated recombination, leaving an allele expressing a tri-cistronic gene (cyclin-dependent kinase 1 + thymidine kinase + mCherry) under the control of the host gene promoter. Cells expressing TK die when exposed to ganciclovir (GCV). This allele was targeted to ES cells that were heterozygous for the Cdk1^em1Nagy allele to create homozygous mutant mice. (J:268168)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk1 Mutation: 21 strains or lines available

References

• Original: J:268168 Liang Q, et al., Linking a cell-division gene and a suicide gene to define and improve cell therapy safety. Nature. 2018 Nov;563(7733):701-704
• All: 1 reference(s)