Nrf1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nrf1^{tm1c(KOMP)Wtsi}
• Name: nuclear respiratory factor 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6272010
• Synonyms: Nrf1^flox
• Gene: Nrf1 Location: Chr6:30047987-30153457 bp, + strand Genetic Position: Chr6, 12.47 cM
• Alliance: Nrf1^{tm1c(KOMP)Wtsi} page
• IMPC: Nrf1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267504
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 30094851 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 30095743. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:267504)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nrf1 Mutation: 66 strains or lines available

References

• Original: J:267504 Kiyama T, et al., Essential roles of mitochondrial biogenesis regulator Nrf1 in retinal development and homeostasis. Mol Neurodegener. 2018 Oct 17;13(1):56
• All: 4 reference(s)