Tiparp^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Tiparp^{tm1d(EUCOMM)Wtsi}
• Name: TCDD-inducible poly(ADP-ribose) polymerase; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6271998
• Synonyms: Tiparp^Ex3, Tiparp^tm1d
• Gene: Tiparp Location: Chr3:65435868-65462939 bp, + strand Genetic Position: Chr3, 30.2 cM
• Alliance: Tiparp^{tm1d(EUCOMM)Wtsi} page
• IMPC: Tiparp gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:268039
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 65453198 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 65454080. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:268039)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tiparp Mutation: 43 strains or lines available

References

• Original: J:268039 Hutin D, et al., Hepatocyte-Specific Deletion of TIPARP, a Negative Regulator of the Aryl Hydrocarbon Receptor, Is Sufficient to Increase Sensitivity to Dioxin-Induced Wasting Syndrome. Toxicol Sci. 2018 Oct 1;165(2):347-360
• All: 3 reference(s)