Tg(CAG-GCH1)#Wlf
Transgene Detail

Summary

• Symbol: Tg(CAG-GCH1)#Wlf
• Name: transgene insertion, Clifford J Woolf
• MGI ID: MGI:6269989
• Synonyms: GCH1-HA
• Transgene: Tg(CAG-GCH1)#Wlf Location: unknown
• Alliance: Tg(CAG-GCH1)#Wlf page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Conditional ready, Epitope tag, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(CAG-GCH1)#Wlf expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GCH1 (2643)

  Gch1	(MGI:95675)

  human cDNA with HA tag

• Mutation details: The transgene contains the following elements: the CAG promoter, a loxP site, a neomycin resistance gene (including stop codon and poly(A) signal) a second loxP site, the human GCH1 cDNA with HA tag sequence coupled to it, and poly(A) signal sequence. This creates a conditional-ready allele that will express HA-tagged human GCH1 only after cre-mediated removal of the neo cassette. (J:223362)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:223362 Latremoliere A, et al., Reduction of Neuropathic and Inflammatory Pain through Inhibition of the Tetrahydrobiopterin Pathway. Neuron. 2015 Jun 17;86(6):1393-406
• All: 4 reference(s)