Rnaseh2b^tm2Hgu
Targeted Allele Detail

Summary

• Symbol: Rnaseh2b^tm2Hgu
• Name: ribonuclease H2, subunit B; targeted mutation 2, MRC Human Genetics Unit
• MGI ID: MGI:6266907
• Synonyms: Rnaseh2b^A174T, Rnaseh2b^{tm2-hgu-A174T}
• Gene: Rnaseh2b Location: Chr14:62569517-62610445 bp, + strand Genetic Position: Chr14, 33.19 cM, cytoband C3
• Alliance: Rnaseh2b^tm2Hgu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231690
• Parent Cell Line: E14TG2aIV (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A neomycin cassette flanked by loxP sites was inserted between exon 6 and 7, generating a targeting vector with two external homology arms of 2.8 kb and 1.7 kb. A point mutation c.520G to A (A174T) was inserted into exon 7 of mouse the gene by site directed mutagenesis. (J:231690)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnaseh2b Mutation: 43 strains or lines available

References

• Original: J:231690 Mackenzie KJ, et al., Ribonuclease H2 mutations induce a cGAS/STING-dependent innate immune response. EMBO J. 2016 Apr 15;35(8):831-44
• All: 2 reference(s)