Rnaseh2b^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Rnaseh2b^{tm1d(EUCOMM)Wtsi}
• Name: ribonuclease H2, subunit B; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6266903
• Gene: Rnaseh2b Location: Chr14:62569517-62610445 bp, + strand Genetic Position: Chr14, 33.19 cM, cytoband C3
• Alliance: Rnaseh2b^{tm1d(EUCOMM)Wtsi} page
• IMPC: Rnaseh2b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231690
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The L1L2_gt0 cassette was inserted at position 62590506 of Chromosome 14 upstream of the critical exon 5 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 5 at position 62591370. The critical exon 5 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:231690)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rnaseh2b Mutation: 43 strains or lines available

References

• Original: J:231690 Mackenzie KJ, et al., Ribonuclease H2 mutations induce a cGAS/STING-dependent innate immune response. EMBO J. 2016 Apr 15;35(8):831-44
• All: 2 reference(s)