Bace1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Bace1^{tm1c(EUCOMM)Hmgu}
• Name: beta-site APP cleaving enzyme 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6258426
• Synonyms: BACE1^fl
• Gene: Bace1 Location: Chr9:45749878-45775694 bp, + strand Genetic Position: Chr9, 25.22 cM
• Alliance: Bace1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Bace1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266958
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 45763695 of Chromosome 9 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 45764551. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele, removing the lacZ and neo cassettes. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:266958)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Bace1 Mutation: 30 strains or lines available

References

• Original: J:266958 Ou-Yang MH, et al., Axonal organization defects in the hippocampus of adult conditional BACE1 knockout mice. Sci Transl Med. 2018 Sep 19;10(459)
• All: 2 reference(s)