Tg(Thy1-YWHAQ)M5Yaco
Transgene Detail

Summary

• Symbol: Tg(Thy1-YWHAQ)M5Yaco
• Name: transgene insertion M5, Talene A Yacoubian
• MGI ID: MGI:6257905
• Synonyms: 14-3-3theta transgenic
• Transgene: Tg(Thy1-YWHAQ)M5Yaco Location: unknown
• Alliance: Tg(Thy1-YWHAQ)M5Yaco page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Epitope tag, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Thy1-YWHAQ)M5Yaco expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  YWHAQ (10971)

  Ywhaq

  (MGI:891963)

  mouse promotor driving human gene with C-terminal HA tag

• Mutation details: The human gene, with sequence for a C-terminal HA tag, was cloned downstream of the mouse promoter to drive neuronal expression. Expression of the tagged gene in the brain of pups was confirmed by both immunohistochemistry and western blotting. Of the two transgene expressing mouse lines, this line has the highest expression and was used for experiments. (J:227621)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:227621 Lavalley NJ, et al., 14-3-3 Proteins regulate mutant LRRK2 kinase activity and neurite shortening. Hum Mol Genet. 2016 Jan 1;25(1):109-22
• All: 4 reference(s)