Ndc80^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ndc80^em1(IMPC)J
• Name: NDC80 kinetochore complex component; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:6256533
• Gene: Ndc80 Location: Chr17:71803095-71833852 bp, - strand Genetic Position: Chr17, 41.87 cM, cytoband E2
• Alliance: Ndc80^em1(IMPC)J page
• IMPC: Ndc80 gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences ATAGGTAAGATAAGAACCAT and GTATAAGAGTATTTAAGGAG, which resulted in a 364 bp deletion beginning at Chromosome 17 position 71,518,724 bp and ending after 71,519,087 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000137722 (exon 6) and 261 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 159 and early truncation 4 amino acids later. (J:188991)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ndc80 Mutation: 36 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 2 reference(s)