Gabrg2^em1Menn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gabrg2^em1Menn
• Name: gamma-aminobutyric acid type A receptor, subunit gamma 2; endonuclease-mediated mutation 1, Steven Mennerick
• MGI ID: MGI:6209490
• Synonyms: gamma2* KI
• Gene: Gabrg2 Location: Chr11:41801030-41891684 bp, - strand Genetic Position: Chr11, 24.8 cM
• Alliance: Gabrg2^em1Menn page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Codon 269 was targeted with single-stranded oligodeoxynucleotide (ssODN) donor DNAs, sgRNAs and Cas9 using CRISPR/Cas9 technology. All components were microinjected into the cytoplasm and pronuclei of fertilized eggs. The resulting p.T269Y (p.Thr269Tyr) mutation renders the receptor picrotoxin (PTX) resistant without affecting basal receptor function. (J:265920)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabrg2 Mutation: 40 strains or lines available

References

• Original: J:265920 Sun MY, et al., Chemogenetic Isolation Reveals Synaptic Contribution of delta GABAA Receptors in Mouse Dentate Granule Neurons. J Neurosci. 2018 Sep 19;38(38):8128-8145
• All: 2 reference(s)