Nfasc^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Nfasc^{tm1c(EUCOMM)Hmgu}
• Name: neurofascin; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6203061
• Gene: Nfasc Location: Chr1:132492428-132669535 bp, - strand Genetic Position: Chr1, 57.42 cM
• Alliance: Nfasc^{tm1c(EUCOMM)Hmgu} page
• IMPC: Nfasc gene page

Mutation origin

• Mutant Cell Line: HEPD0529_1_B03
• Germline Transmission: Earliest citation of germline transmission: J:240865
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 132561680 of Chromosome 1 upstream of the critical exons 6 and 7 (Build GRCm39). The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, an SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 132563028. Exon 6 and 7 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065, J:240865)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nfasc Mutation: 59 strains or lines available

References

• Original: J:240865 Amor V, et al., The paranodal cytoskeleton clusters Na+ channels at nodes of Ranvier. Elife. 2017 Jan 30;6:e21392
• All: 2 reference(s)