Siglecg^tm2.1Lnit
Targeted Allele Detail

Summary

• Symbol: Siglecg^tm2.1Lnit
• Name: sialic acid binding Ig-like lectin G; targeted mutation 2.1, Lars Nitschke
• MGI ID: MGI:6202624
• Synonyms: Siglec-G-R120E
• Gene: Siglecg Location: Chr7:43057623-43067773 bp, + strand Genetic Position: Chr7, 28.25 cM
• Alliance: Siglecg^tm2.1Lnit page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:265415
• Parent Cell Line: BALB/c-I (ES Cell)
• Strain of Origin: BALB/cJ

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: An arginine to glutamic acid (R120E) mutation was inserted in exon 3 and a floxed neo cassette was inserted in intron 4 via homologous recombination. Cre-mediated recombination removed the neo cassette. The exon 3 mutation changes a conserved positively charged amino acid in the first ligand binding domain to a negatively charged amino acid. The mutant protein shows surface expression in B-cells similar to wild-type protein but fails to bind ligand. (J:265415)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Siglecg Mutation: 39 strains or lines available

References

• Original: J:265415 Hutzler S, et al., The ligand-binding domain of Siglec-G is crucial for its selective inhibitory function on B1 cells. J Immunol. 2014 Jun 1;192(11):5406-14
• All: 2 reference(s)