Gt(ROSA)26Sor^{tm1(CAG-EGFP)Maito}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-EGFP)Maito}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Mamoru Ito
• MGI ID: MGI:6202418
• Synonyms: VloxP-EGFP KI
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-EGFP)Maito} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:263660
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6JJic

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A construct containing the CAG promoter with a VloxP-STOP-VloxP cassette preceding EGFP sequence was inserted into the intron of the Gt(ROSA)26Sor locus via homologous recombination in Wit 9 ES cells. VCre-mediated recombination of this allele results in the expression of the EGFP reporter driven by the CAG promoter. VCre/VloxP is a site-specific recombination system, which was derived from plasmid p0908 of Vibrio sp. VCre is a recombinase that recognizes its own specific target sites (VloxP). (J:263660)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1093 strains or lines available

Notes

ES cell line = Wit 9 (C57BL/6JJic).

References

• Original: J:263660 Yoshimura Y, et al., Novel reporter and deleter mouse strains generated using VCre/VloxP and SCre/SloxP systems, and their system specificity in mice. Transgenic Res. 2018 Apr;27(2):193-201
• All: 1 reference(s)