Crh^tm1Jde
Targeted Allele Detail

Summary

• Symbol: Crh^tm1Jde
• Name: corticotropin releasing hormone; targeted mutation 1, Jan Deussing
• MGI ID: MGI:6201412
• Synonyms: Crh^tZ
• Gene: Crh Location: Chr3:19747565-19749560 bp, - strand Genetic Position: Chr3, 5.75 cM
• Alliance: Crh^tm1Jde page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264526
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: A construct, which were flanked by attP sites, contained (5' to 3') upstream loxP site, first frt site, adenovirus splice acceptor (SA), tau-lacZ (tZ) reporter gene equipped at its C terminus with a flag tag, second frt site and a reverse-oriented EM7-neo positive selection cassette, including a bovine growth hormone polyadenylation signal. Downstream of the second attP site there was a reverse-oriented Pgk1 promoter, a third frt site all inserted into upstream of exon 2 into intron 1. A downstream loxP site was inserted into exon 2 downstream of 3' UTR. (J:264526)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crh Mutation: 39 strains or lines available

References

• Original: J:264526 Dedic N, et al., Chronic CRH depletion from GABAergic, long-range projection neurons in the extended amygdala reduces dopamine release and increases anxiety. Nat Neurosci. 2018 Jun;21(6):803-807
• All: 1 reference(s)