Pik3ca^tm1Mawa
Targeted Allele Detail

Summary

• Symbol: Pik3ca^tm1Mawa
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; targeted mutation 1, Matthew Warman
• MGI ID: MGI:6201353
• Synonyms: Pik3ca:C420R
• Gene: Pik3ca Location: Chr3:32451203-32520256 bp, + strand Genetic Position: Chr3, 15.7 cM, cytoband B
• Alliance: Pik3ca^tm1Mawa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting construct is designed to introduce a C420R point mutation (T2928C; TGT - CGT) into exon 8 of the gene with flanking loxP sites in introns 7 and 9. An FRT-flanked cassette containing the engrailed-2 strong splice acceptor and a reverse-orientation neomycin cassette under the control of the PGK promoter was also inserted immediately downstream of the intron 9 loxP site. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pik3ca Mutation: 66 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)