Pax1^tm3.1Tlu
Targeted Allele Detail

Summary

• Symbol: Pax1^tm3.1Tlu
• Name: paired box 1; targeted mutation 3.1, Thomas Lufkin
• MGI ID: MGI:6200898
• Synonyms: Pax1^{F2A-EGFP:Pax1+}
• Gene: Pax1 Location: Chr2:147203850-147216972 bp, + strand Genetic Position: Chr2, 72.63 cM
• Alliance: Pax1^tm3.1Tlu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:239547
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (No functional change, Reporter)
• Mutation: Insertion
• Mutation details: An RAKR furin motif sequence to trim the residual 2A peptide from the upstream protein, glycine-serine-glycine (GCG) spacer, foot-and-mouth disease virus 2A peptide (F2A) sequence, EGFP and FRT-flanked neomycin resistance (neo) cassette were inserted in frame immediately before the STOP codon at exon 5. Flp-mediated recombination removed the selection cassette. (J:239547)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pax1 Mutation: 26 strains or lines available

References

• Original: J:239547 Sivakamasundari V, et al., A developmental transcriptomic analysis of Pax1 and Pax9 in embryonic intervertebral disc development. Biol Open. 2017 Feb 15;6(2):187-199
• All: 1 reference(s)