Cacna2d4^em1Leea
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cacna2d4^em1Leea
• Name: calcium channel, voltage-dependent, alpha 2/delta subunit 4; endonuclease-mediated mutation 1, Amy Lee
• MGI ID: MGI:6200657
• Synonyms: alpha2delta-4 KO
• Gene: Cacna2d4 Location: Chr6:119213487-119329368 bp, + strand Genetic Position: Chr6, 56.62 cM
• Alliance: Cacna2d4^em1Leea page

Mutation origin

• Strain of Origin: C57BL/6J x (C57BL/6J x SJL/J)F1/J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Exon 2 was targeted using two sgRNAs and Cas9n (nickase version of Cas9). The result was an out-of-frame deletion (c.360_408del) that leads to a premature translation stop codon after 14 novel codons. (J:265366)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cacna2d4 Mutation: 76 strains or lines available

References

• Original: J:265366 Kerov V, et al., alpha2delta-4 Is Required for the Molecular and Structural Organization of Rod and Cone Photoreceptor Synapses. J Neurosci. 2018 Jul 4;38(27):6145-6160
• All: 4 reference(s)