About   Help   FAQ
Transgene Detail
Symbol: Tg(CAG-mCherry/Plk4)#Reba
Name: transgene insertion, Renata Basto
MGI ID: MGI:6200620
Synonyms: Tg(mChPlk4)
Transgene: Tg(CAG-mCherry/Plk4)#Reba  Location: unknown  
Strain of Origin:  C57BL/6N
Transgene Type:    Transgenic (Conditional ready, Inserted expressed sequence, Reporter)
Mutation:    Insertion
Tg(CAG-mCherry/Plk4)#Reba expresses 1 gene
Mutation detailsThe transgene construct comprises the following elements: the CAG promoter, A loxP site, a chloramphenicol acetyl transferase gene and STOP cassette, a second loxP site, the mCherry red fluorescent gene fused in-frame (via a Gly-Gly-Ser linker) with the mouse polo like kinase 4 cDNA, and SV40 poly(A) signal sequence. The construct was microinjected into the pronuclei of fertilized oocytes. This creates a conditional-ready allele where the inserted chimeric reporter-kinase sequence will only be expressed after cre-mediated excission of the floxed CAT gene. (J:198922)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Original:  J:198922 Marthiens V, et al., Centrosome amplification causes microcephaly. Nat Cell Biol. 2013 Jul;15(7):731-40
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.14
The Jackson Laboratory