Ctcf^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ctcf^{tm1c(EUCOMM)Wtsi}
• Name: CCCTC-binding factor; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6199123
• Synonyms: Ctcf^fl
• Gene: Ctcf Location: Chr8:106363200-106409554 bp, + strand Genetic Position: Chr8, 53.04 cM
• Alliance: Ctcf^{tm1c(EUCOMM)Wtsi} page
• IMPC: Ctcf gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264983
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt1 cassette was inserted at position 106401044 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 106402005. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:264983)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ctcf Mutation: 78 strains or lines available

References

• Original: J:264983 Shin JO, et al., CTCF Regulates Otic Neurogenesis via Histone Modification in the Neurog1 Locus. Mol Cells. 2018 Jul 31;41(7):695-702
• All: 7 reference(s)