Akap5^tm3.1Mdaq
Targeted Allele Detail

Summary

• Symbol: Akap5^tm3.1Mdaq
• Name: A kinase anchor protein 5; targeted mutation 3.1, Mark Dell'Acqua
• MGI ID: MGI:6198520
• Synonyms: AKAP150CS, Akap5CS
• Gene: Akap5 Location: Chr12:76371665-76380927 bp, + strand Genetic Position: Chr12, 33.73 cM
• Alliance: Akap5^tm3.1Mdaq page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:269375
• Parent Cell Line: EC7.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129X1/SvJ)F1

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Nucleotide substitutions
• Mutation details: The Akap5CS mutation eliminates palmitoylation of AKAP150 by mutating cysteines 36 and 123 to serines. These mutations were introduced into the single coding exon of an Akap5 genomic DNA fragment via piggyBac (PB) transposon based method from a C57BL/6 BAC clone. In this targeting vector, the CS mutation and a C-terminal myc-epitope tag were introduced by piggyBac method with a neomycin resistance cassette flanked by the 3 and 5 long-terminal repeat (LTR) of PB inserted within the Akap5 exon. The neomycin resistance cassette was then removed from the targeted locus by remobilizing the PB with transient expression of PB transposase. (J:269375)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Akap5 Mutation: 27 strains or lines available

References

• Original: J:269375 Purkey AM, et al., AKAP150 Palmitoylation Regulates Synaptic Incorporation of Ca(2+)-Permeable AMPA Receptors to Control LTP. Cell Rep. 2018 Oct 23;25(4):974-987.e4
• All: 1 reference(s)