Paqr3^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Paqr3^{tm1c(KOMP)Wtsi}
• Name: progestin and adipoQ receptor family member III; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6198332
• Synonyms: Paqr3^flox
• Gene: Paqr3 Location: Chr5:97230188-97259455 bp, - strand Genetic Position: Chr5, 47.29 cM
• Alliance: Paqr3^{tm1c(KOMP)Wtsi} page
• IMPC: Paqr3 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264679
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt2 cassette was inserted at position 97255738 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 97256643. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:264679)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Paqr3 Mutation: 22 strains or lines available

References

• Original: J:264679 Zhao Z, et al., Hepatic PPARalpha function is controlled by polyubiquitination and proteasome-mediated degradation through the coordinated actions of PAQR3 and HUWE1. Hepatology. 2018 Jul;68(1):289-303
• All: 2 reference(s)