Spef2^tm1.1Siro
Targeted Allele Detail

Summary

• Symbol: Spef2^tm1.1Siro
• Name: sperm flagellar 2; targeted mutation 1.1, Anu Sironen
• MGI ID: MGI:6197576
• Gene: Spef2 Location: Chr15:9578279-9748954 bp, - strand Genetic Position: Chr15, 4.24 cM
• Alliance: Spef2^tm1.1Siro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242894
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: An FRT flanked DsRed gene and stop codon and loxP site were inserted upstream of exon 3 and a loxP site was inserted downstream of exon 5 via homologous recombination. Flp mediated recombination removed the DsRed gene and stop codon leaving exons 3 - 5 floxed. (J:242894)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spef2 Mutation: 111 strains or lines available

References

• Original: J:242894 Lehti MS, et al., SPEF2 functions in microtubule-mediated transport in elongating spermatids to ensure proper male germ cell differentiation. Development. 2017 Jul 15;144(14):2683-2693
• All: 2 reference(s)