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Mecp2tm7.1Bird
Targeted Allele Detail
Summary
Symbol: Mecp2tm7.1Bird
Name: methyl CpG binding protein 2; targeted mutation 7.1, Adrian Bird
MGI ID: MGI:6196350
Synonyms: MeCP2 deltaNC
Gene: Mecp2  Location: ChrX:73070198-73129296 bp, - strand  Genetic Position: ChrX, 37.63 cM
Alliance: Mecp2tm7.1Bird page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:252845
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA targeting vector was used to modify the locus as follows: a loxP site, a neomycin resistance gene cassette, a transcriptional STOP cassette and a second loxP site, all inserted into intron 2, a modified exon 3, a modified exon 4, sequence for linker peptide GSSGSSG, and the EGFP gene. The modification to exon 3 is the deletion of sequence for codons 30-87 (splice variant e1) or 13-70 (splice variant e2). The modification to exon 4 is the deletion of sequence for codons 313-484 (approximately)(splice variant e2). The neo-STOP cassette was subsequently removed through cre-mediated recombination. This leaves a knockin allele where the endogenous gene is replaced with a truncated version (with sequences immediately upstream of the methyl-CpG binding domain (MBD) and downstream of the NCor/SMRT interaction domain (NID) deleted) fused to a fluorescent marker. The truncated protein retains 52% of the wild-type sequence. (J:252845)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mecp2 Mutation:  43 strains or lines available
References
Original:  J:252845 Tillotson R, et al., Radically truncated MeCP2 rescues Rett syndrome-like neurological defects. Nature. 2017 Oct 19;550(7676):398-401
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory