Mecp2tm7.1Bird
Targeted Allele Detail
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Symbol: |
Mecp2tm7.1Bird |
Name: |
methyl CpG binding protein 2; targeted mutation 7.1, Adrian Bird |
MGI ID: |
MGI:6196350 |
Synonyms: |
MeCP2 deltaNC |
Gene: |
Mecp2 Location: ChrX:73070198-73129296 bp, - strand Genetic Position: ChrX, 37.63 cM
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Alliance: |
Mecp2tm7.1Bird page
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Germline Transmission: |
Earliest citation of germline transmission:
J:252845
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Parent Cell Line: |
Not Specified (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Reporter) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: A targeting vector was used to modify the locus as follows: a loxP site, a neomycin resistance gene cassette, a transcriptional STOP cassette and a second loxP site, all inserted into intron 2, a modified exon 3, a modified exon 4, sequence for linker peptide GSSGSSG, and the EGFP gene. The modification to exon 3 is the deletion of sequence for codons 30-87 (splice variant e1) or 13-70 (splice variant e2). The modification to exon 4 is the deletion of sequence for codons 313-484 (approximately)(splice variant e2). The neo-STOP cassette was subsequently removed through cre-mediated recombination. This leaves a knockin allele where the endogenous gene is replaced with a truncated version (with sequences immediately upstream of the methyl-CpG binding domain (MBD) and downstream of the NCor/SMRT interaction domain (NID) deleted) fused to a fluorescent marker. The truncated protein retains 52% of the wild-type sequence.
(J:252845)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Mecp2 Mutation: |
43 strains or lines available
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Original: |
J:252845 Tillotson R, et al., Radically truncated MeCP2 rescues Rett syndrome-like neurological defects. Nature. 2017 Oct 19;550(7676):398-401 |
All: |
1 reference(s) |
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