Prlr^tm1.1Darg
Targeted Allele Detail

Summary

• Symbol: Prlr^tm1.1Darg
• Name: prolactin receptor; targeted mutation 1.1, David R Grattan
• MGI ID: MGI:6196142
• Synonyms: Prlr^lox
• Gene: Prlr Location: Chr15:10177324-10349266 bp, + strand Genetic Position: Chr15, 5.23 cM
• Alliance: Prlr^tm1.1Darg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235209
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A lox66 site was inserted in intron 4, and, in oposite transcriptional direction, a poly(A) signal, the EGFP fluorescent marker gene, and the 3' part of intron 4 (to include exon 5 splice acceptor). This was followed by a lox71 site. The FRT site flanked neomycin resistance gene cassette that was also inserted was subsequently removed through flp-mediated recombination. This creates a conditional-ready allele where after cre-mediated recombination the sequence between the mutated lox sites is inverted. In that recombined allele exon 4 would splice to the EGFP gene and transcription would terminate at the poly(A) signal downstream of the marker gene, effectively deleting all the downstream exons (5-10). This chimeric transcript lacks sequence for the entire transmembrane and intracellular portions of the encoded receptor, resulting in a complete absence of both long and short isoforms. (J:235209)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prlr Mutation: 42 strains or lines available

References

• Original: J:235209 Brown RS, et al., Conditional Deletion of the Prolactin Receptor Reveals Functional Subpopulations of Dopamine Neurons in the Arcuate Nucleus of the Hypothalamus. J Neurosci. 2016 Aug 31;36(35):9173-85
• All: 13 reference(s)