Zfyve28^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Zfyve28^{tm1c(EUCOMM)Wtsi}
• Name: zinc finger, FYVE domain containing 28; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6195793
• Gene: Zfyve28 Location: Chr5:34352237-34445793 bp, - strand Genetic Position: Chr5, 17.85 cM
• Alliance: Zfyve28^{tm1c(EUCOMM)Wtsi} page
• IMPC: Zfyve28 gene page

Mutation origin

• Mutant Cell Line: EPD0431_1_B06
• Germline Transmission: Earliest citation of germline transmission: J:262762
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34391339 of Chromosome 5 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 34392306. The critical exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:262762)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zfyve28 Mutation: 60 strains or lines available

References

• Original: J:262762 Zambrano S, et al., FYVE domain-containing protein ZFYVE28 regulates EGFR-signaling in podocytes but is not critical for the function of filtration barrier in mice. Sci Rep. 2018 Mar 16;8(1):4712
• All: 1 reference(s)