Hoxd3^em1Ddu
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hoxd3^em1Ddu
• Name: homeobox D3; endonuclease-mediated mutation 1, Denis Duboule
• MGI ID: MGI:6195703
• Synonyms: Hoxd3^hd
• Gene: Hoxd3 Location: Chr2:74542337-74578615 bp, + strand Genetic Position: Chr2, 44.13 cM, cytoband C2
• Alliance: Hoxd3^em1Ddu page

Mutation origin

• Strain of Origin: Not Specified

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A 67 bp deletion was created using sgRNAs 5'-GTACGCCGTGCGCACCCTCT-3' and 5'-GGCGCGGCCGGCACAGATAG-3' and CRISPR/Cas9 technology. This deletion in the second exon crates a frameshift and premature stop codon. As a result virtually the entire homeodomain is eliminated. (J:252916)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxd3 Mutation: 13 strains or lines available

References

• Original: J:252916 Zakany J, et al., Control of growth and gut maturation by HoxD genes and the associated lncRNA Haglr. Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):E9290-E9299
• All: 1 reference(s)