Sik2^tm1.1Arte
Targeted Allele Detail

Summary

• Symbol: Sik2^tm1.1Arte
• Name: salt inducible kinase 2; targeted mutation 1.1, TaconicArtemis
• MGI ID: MGI:6195669
• Synonyms: Sik2^ki
• Gene: Sik2 Location: Chr9:50804101-50920373 bp, - strand Genetic Position: Chr9, 27.75 cM
• Alliance: Sik2^tm1.1Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246310
• Parent Cell Line: ART B6 3.6 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A threonine to alanine change at position 175 (T175A) was introduced resulting in a kinase dead knock-in mutation. LoxP sites were introduced 3' and 5' to the targeted exons. A F3-flanked puromycin and FRT-flanked neomycin selection cassettes were removed via Flp-mediated recombination. (J:246310)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sik2 Mutation: 46 strains or lines available

References

• Original: J:246310 Darling NJ, et al., Inhibition of SIK2 and SIK3 during differentiation enhances the anti-inflammatory phenotype of macrophages. Biochem J. 2017 Feb 15;474(4):521-537
• All: 3 reference(s)