Spmip8^tm1Osb
Targeted Allele Detail

Summary

• Symbol: Spmip8^tm1Osb
• Name: sperm microtubule inner protein 8; targeted mutation 1, Research Institute for Microbial Diseases, Osaka University
• MGI ID: MGI:6194911
• Synonyms: Tepp^{tm1a(KOMP)Osb}
• Gene: Spmip8 Location: Chr8:96038148-96053239 bp, + strand Genetic Position: Chr8, 47.12 cM, cytoband C5
• Alliance: Spmip8^tm1Osb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234235
• Parent Cell Line: G101 (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 96045822 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 96048189. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:234235)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spmip8 Mutation: 14 strains or lines available

References

• Original: J:234235 Miyata H, et al., Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7704-10
• All: 1 reference(s)