About   Help   FAQ
TcraLn1Bdb
Allele Detail
Nomenclature
Symbol: TcraLn1Bdb
Name: T cell receptor alpha chain; recombination line 1, Brian Brown
MGI ID: MGI:6194869
Gene: Tcra  Location: unknown  Genetic Position: Chr14, cytoband D1-D2
Mutation
origin
Strain of Origin:  (BALB/cJ x C57BL/6J)F1
Mutation
description
Allele Type:    Other (Not Specified)
Mutation:    Other
 
Mutation detailsThis allele is generated using somatic cell nuclear transfer (SCNT). First, a lentivirus encoding GFP was injected into (BALB/cJ x C57BL/6J)F1 mice (CB6F1; H-2K). After 2 weeks, GFP-200-208 H-2K tetramer<+> CD8<+> T cells were isolated. The T cell nucleus was injected into an enucleated oocyte, and the resulting cell was used to generate embryonic stem (ES) cells. ES cells were injected into recipient blastocysts, and blastocysts implanted into pseudopregnant females. PCR analysis and Sanger sequencing reveal that the rearranged TCR is Valpha1-J30 (trav7-4, traj30, and trac). (J:248646)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tcra Mutation:  92 strains or lines available
References
Original:  J:248646 Agudo J, et al., GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell interactions. Nat Biotechnol. 2015 Dec;33(12):1287-1292
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
11/23/2021
MGI 6.17
The Jackson Laboratory