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Allele Detail
Symbol: TcrbLn1Bdb
Name: T cell receptor beta chain; recombination line 1, Brian Brown
MGI ID: MGI:6194858
Gene: Tcrb  Location: unknown  Genetic Position: Chr6, cytoband A-C
Strain of Origin:  (BALB/cJ x C57BL/6J)F1
Allele Type:    Other (Not Specified)
Mutation:    Other
Mutation detailsThis allele is generated using somatic cell nuclear transfer (SCNT). First, a lentivirus encoding GFP was injected into (BALB/cJ x C57BL/6J)F1 mice (CB6F1; H-2K). After 2 weeks, GFP-200-208- H-2K tetramer<+> CD8<+> T cells were isolated. The T cell nucleus was injected into an enucleated oocyte, and the resulting cell was used to generate embryonic stem (ES) cells. ES cells were injected into recipient blastocysts, and blastocysts implanted into pseudopregnant females. PCR analysis and Sanger sequencing reveal that the rearranged TCR is Vbeta4-D1-J1.6-C1 (trbv2-trbj1.6-trbc1). (J:248646)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tcrb Mutation:  90 strains or lines available
Original:  J:248646 Agudo J, et al., GFP-specific CD8 T cells enable targeted cell depletion and visualization of T-cell interactions. Nat Biotechnol. 2015 Dec;33(12):1287-1292
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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