Megf10^{tm1b(KOMP)Jrs}
Targeted Allele Detail

Summary

• Symbol: Megf10^{tm1b(KOMP)Jrs}
• Name: multiple EGF-like-domains 10; targeted mutation 1b, Joshua R Sanes
• MGI ID: MGI:6194030
• Synonyms: Megf10^lacZ
• Gene: Megf10 Location: Chr18:57266162-57430539 bp, + strand Genetic Position: Chr18, 31.5 cM
• Alliance: Megf10^{tm1b(KOMP)Jrs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:182578
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting cassette obtained from KOMP is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4. The critical exon 4 is thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:182578)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Megf10 Mutation: 71 strains or lines available

References

• Original: J:182578 Kay JN, et al., MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons. Nature. 2012 Mar 22;483(7390):465-9
• All: 5 reference(s)