Tg(PRNP)1534Gcte
Transgene Detail

Summary

• Symbol: Tg(PRNP)1534Gcte
• Name: transgene insertion 1534, Glenn C Telling
• MGI ID: MGI:6193854
• Synonyms: Tg(CerPrP)1534
• Transgene: Tg(PRNP)1534Gcte Location: unknown
• Alliance: Tg(PRNP)1534Gcte page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(PRNP)1534Gcte expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  deer, mule	Prnp	Syrian hamster promoter and mule deer gene fragment

• Mutation details: The partial open reading frame of the S2 allele (GenBank accession AF009180) of Odocoileus hemionus hemionus or mule deer prion protein was inserted into the cosSHa.Tet cosmid expression vector, replacing the tetracycline resistance gene. This expression vector contains a 49 kb DNA fragment encompassing the Syrian hamster Prnp gene, including its promoter, with the coding region in exon 2 replaced with a tetracycline resistance gene. To increase transgene expression, the transgene nucleotide sequence was modified by site-directed mutagenesis to produce a consensus Kozak translation initiation sequence immediately upstream of the initiating ATG. Two founders, 1534 and 1536, were generated by microinjection of the transgene into fertilized embryos from Prnp knockout mice Prnp^tm1Cwe on an FVB/N background. (J:94219)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:94219 Browning SR, et al., Transmission of prions from mule deer and elk with chronic wasting disease to transgenic mice expressing cervid PrP. J Virol. 2004 Dec;78(23):13345-50
• All: 1 reference(s)