Tg(KRT14)1Efu
Transgene Detail

Summary

• Symbol: Tg(KRT14)1Efu
• Name: transgene insertion 1, Elaine Fuchs
• MGI ID: MGI:6192717
• Synonyms: K14-P
• Transgene: Tg(KRT14)1Efu Location: unknown
• Alliance: Tg(KRT14)1Efu page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(KRT14)1Efu expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  KRT14 (3861)

  Krt14

  (MGI:96688)

  human promoter and gene

• Mutation details: The transgene comprises the following elements, all from human keratin 14: upstream sequence (from the HindIII site 2.5 kb upstream of the transcription start site) including the promoter, the cDNA with the antigenic portion of neuropeptide tachykinin 1 (substance P) inserted in-frame 18 bp upstream of the stop codon, and 3' UTR sequence including poly(A) signal (up to the EcoRI site 700 bp downstream of poly(A) signal). The construct was micro-injected into the male pronuclei of fertilized oocytes. Immunohistological analysis using substance P antibody shows expression in the basal layer of the epidermis and in the outer root sheath cells of the hair follicles. Line 1 showed consistent (non-chimeric) expression of the transgene. (J:113642)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:113642 Vassar R, et al., Tissue-specific and differentiation-specific expression of a human K14 keratin gene in transgenic mice. Proc Natl Acad Sci U S A. 1989 Mar;86(5):1563-7
• All: 1 reference(s)