Gt(ROSA)26Sor^{tm2.1(CAG-EGFP*)Harv}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(CAG-EGFP*)Harv}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Brandon Harvey
• MGI ID: MGI:6192348
• Synonyms: Gt(ROSA)26Sor^{tm2.1(GCaMPer)Harv}, Rosa26-GCaMPer, Rosa26-LSL-GCaMPer
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(CAG-EGFP*)Harv} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:361427
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct introduced into the locus a CAG promoter, floxed stop sequence, and endoplasmic reticulum-localized low affinity variant EGFP-based calcium censor (GCaMPer). The GCaMP3 fusion gene contains a cDNA sequence encoding the chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP (amino acid residues 149-238 followed by amino acid residues 1-144 (including M153K, V163A, S175G, D180Y, T203V, A206K, and V93I mutations to increase dynamic range and baseline fluorescence), and a DNA fragment encoding amino acid residues 2-148 of the rat calmodulin (modified to harbor the N60D mutation to increase the fluorescence change for small calcium transients). Flp-mediated recombination removed the FRT-flanked selection cassette. (J:361427)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1024 strains or lines available

References

• Original: J:361427 Harvey B, Direct Data Submission GCaMPer at Rosa26 locus. MGI Direct Data Submission. 2025
• All: 1 reference(s)