Dlg4^tm6.1Grnt
Targeted Allele Detail

Summary

• Symbol: Dlg4^tm6.1Grnt
• Name: discs large MAGUK scaffold protein 4; targeted mutation 6.1, Seth G N Grant
• MGI ID: MGI:6192330
• Synonyms: PSD95-eGFP, PSD95^eGFP
• Gene: Dlg4 Location: Chr11:69908029-69938107 bp, + strand Genetic Position: Chr11, 42.96 cM, cytoband B4
• Alliance: Dlg4^tm6.1Grnt page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:263617
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The eGFP (enhanced green fluorescent protein) gene was inserted at the 3' end of the target gene just before the stop codon. The loxP site flanked neomycin resistance gene that was inserted downstream of the gene was removed through subsequent cre-mediated recombination. This creates an allele expressing a chimeric protein containing the marker peptide at the C-terminus of the endogenous peptide. (J:263617, J:269345)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Dlg4 Mutation: 52 strains or lines available

References

• Original: J:263617 Broadhead MJ, et al., PSD95 nanoclusters are postsynaptic building blocks in hippocampus circuits. Sci Rep. 2016 Apr 25;6:24626
• All: 8 reference(s)