Ngb^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ngb^{tm1d(EUCOMM)Wtsi}
• Name: neuroglobin; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6191780
• Gene: Ngb Location: Chr12:87144305-87149313 bp, - strand Genetic Position: Chr12, 41.29 cM, cytoband D3
• Alliance: Ngb^{tm1d(EUCOMM)Wtsi} page
• IMPC: Ngb gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258100
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 87146546 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 87148064. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was then created by flp recombinase expression in mice carrying this allele. Subsequent cre expression resulted in a knockout mouse. (J:258100)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ngb Mutation: 9 strains or lines available

References

• Original: J:258100 Nowotny M, et al., Hearing Without Neuroglobin. Neuroscience. 2017 Dec 16;366:138-148
• All: 1 reference(s)