Tbc1d14^em1Iexas
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tbc1d14^em1Iexas
• Name: TBC1 domain family, member 14; endonuclease-mediated mutation 1, Tomoji Mashimo
• MGI ID: MGI:6188987
• Synonyms: floxed mKIAA1322
• Gene: Tbc1d14 Location: Chr5:36647948-36743611 bp, - strand Genetic Position: Chr5, 19.15 cM
• Alliance: Tbc1d14^em1Iexas page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: Exon 3 of the gene was targeted for flanking insertions of loxP sites. lssDNA (long single-stranded DNA) containing sequence for a loxP site, the end of intron 2, the entire exon 3, the start of intron 3 and a second loxP site was microinjected into zygotes together with two sgRNAs and Cas9 mRNA (with 81 bp poly(A) tail appended). This conditional-ready allele was found in founders 1, 2, 3 and 4. (J:261995)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tbc1d14 Mutation: 36 strains or lines available

References

• Original: J:261995 Miyasaka Y, et al., CLICK: one-step generation of conditional knockout mice. BMC Genomics. 2018 May 2;19(1):318
• All: 1 reference(s)