Serpina3n^em1Iexas
Endonuclease-mediated Allele Detail

Summary

• Symbol: Serpina3n^em1Iexas
• Name: serine (or cysteine) peptidase inhibitor, clade A, member 3N; endonuclease-mediated mutation 1, Tomoji Mashimo
• MGI ID: MGI:6188919
• Gene: Serpina3n Location: Chr12:104372988-104380588 bp, + strand Genetic Position: Chr12, 54.17 cM, cytoband F1
• Alliance: Serpina3n^em1Iexas page

Mutation origin

• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready)
• Mutation: Insertion
• Mutation details: Exon 4 of the gene was targeted for flanking insertions of loxP sites. lssDNA (long single-stranded DNA) containing sequence for a loxP site, the end of intron 3, the entire exon 4, the start of intron 4 and a second loxP site was electroporated or microinjected into zygotes together with two sgRNAs and Cas9 mRNA (with 81 bp poly(A) tail appended). This conditional-ready allele was found in some of the F1 offspring of F0 founders 3, 20, 22 and 23. (J:261995)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Serpina3n Mutation: 33 strains or lines available

References

• Original: J:261995 Miyasaka Y, et al., CLICK: one-step generation of conditional knockout mice. BMC Genomics. 2018 May 2;19(1):318
• All: 1 reference(s)