Traj18^tm1.2Tgi
Targeted Allele Detail

Summary

• Symbol: Traj18^tm1.2Tgi
• Name: T cell receptor alpha joining 18; targeted mutation 1.2, Masaru Taniguchi
• MGI ID: MGI:6188629
• Gene: Traj18 Location: Chr14:54438234-54438299 bp, + strand Genetic Position: Chr14, 27.69 cM
• Alliance: Traj18^tm1.2Tgi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249268
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted 3 the J alpha 18 (J alpha 281) gene segment and an FRT-flanked neomycin cassette followed by a second loxP site was placed 3 to the gene segment. The neomycin selection cassette was removed via FLP-mediated recombination. The J alpha 18 segment and neo cassette were removed via cre-mediated recombination of the loxP sites. (J:249268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Traj18 Mutation: 4 strains or lines available

Notes

ES Cell line = B6JN/1 mouse embryonic stem cells, derived from a (C57BL6/J Jcl) x (C57BL6/N Jcl) embryo

References

• Original: J:249268 Dashtsoodol N, et al., Generation of Novel Traj18-Deficient Mice Lacking Valpha14 Natural Killer T Cells with an Undisturbed T Cell Receptor alpha-Chain Repertoire. PLoS One. 2016;11(4):e0153347
• All: 2 reference(s)